Species:

Human

Alternative Name:

B2MG;B2M

Uniport ID:

P61769

Assay Type:

Competitive

unit:

ng/mL

Standard:

2000ng/mL

Sensitivity:

18.75 ng/mL

Detection Range:

31.25-2000ng/mL

Assay Time:

1.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=50μL

Research:

Tumor immunity;Hepatology;

Test Principle:

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human BMG/β2-MG. During the reaction, Human BMG/β2-MG in the sample or standard competes with a fixed amount of Human BMG/β2-MG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Human BMG/β2-MG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human BMG/β2-MG in tested samples can be calculated by comparing the OD of the samples to the standard curve.